Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Rutin has anti-asthmatic effects in an ovalbumin-induced asthmatic mouse model

Hong-yan Lv¹, Jing Chen², Ting Wang³

¹Department of Pediatrics, Shandong Jining No.1 Peopleâ€™s Hospital, Shandong 272000; ²Department of Pediatrics, Affiliated Hospital of Jining Medical University, Xiamen 361000; ³Out-patient Department, Affiliated Hospital of Jining Medical College, Jining 272000, China.

For correspondence:- Ting Wang Email: lhy_angel@163.com Tel:+865373616166

Received: 31 March 2016 Accepted: 14 May 2017 Published: 29 June 2017

Citation: Lv H, Chen J, Wang T. Rutin has anti-asthmatic effects in an ovalbumin-induced asthmatic mouse model. Trop J Pharm Res 2017; 16(6):1337-1347 doi: 10.4314/tjpr.v16i6.18

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the anti-asthmatic effects of the flavonoid rutin in an ovalbumin (OVA)-induced asthmatic mouse model.

Methods: Asthma was induced by OVA induction. Effects of rutin (25–100 mg/kg BW) on interleukins (IL)-4, IL-5, and IL-13 and interferon (IFN)-γ in bronchoalveolar lavage fluid (BALF) and serum OVA-specific IgE levels were assessed by enzyme linked immunosorbent assay (ELISA). Airway hyperresponsiveness (AHR) was measured by determining lung compliance and airway resistance. expression levels of p-I-κBα, p-NF-κB p65, TNF-α, and Foxp3 were also assessed. The CD4⁺ CD25⁺ Foxp3⁺ T regulatory cell population was determined by flow cytometry.

Results: Rutin significantly (p < 0.05) decreased Th2 cytokines (IL-4, IL-5, IL-13), whereas it enhanced IFN-γ levels in BALF. Decreased OVA-specific serum IgE was also observed with improved lung histology. Rutin was effective in reducing AHR and regulating the expression levels of p-I-κBα, p-NF-κB p65, and TNF α, and also increased the CD4⁺ CD25⁺ Foxp3⁺ Treg cell population and Foxp3 mRNA and protein levels.

Conclusion: Rutin effectively suppresses OVA-induced asthma and improves airway function by suppressing inflammatory mediators and inflammatory cell infiltration.

Keywords: Asthma, CD4+ CD25+, Cytokines, Inflammation, Ovalbumin, Rutin, Protein levels